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Primary bile acid biosynthesis

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
27-Hydroxycholesterol + reduced_flavodoxin + O2 <=> 7alpha,27-dihydroxycholesterol + flavin + H2O 1.14.14.23 cholesterol 7alpha-monooxygenase
1.14.14.29 25/26-hydroxycholesterol 7alpha-hydroxylase


cholesterol + reduced_adrenodoxin + H+ + O2 <=> 27-Hydroxycholesterol + oxidized_ferredoxin + H2O 1.14.15.15 cholestanetriol 26-monooxygenase

(25S)-3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA + acceptor <=> (24E)-3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA + reduced_acceptor 1.17.99.3 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA 24-hydroxylase

5beta-cholestane-3alpha,7alpha,12alpha-triol + NADP+ <=> 7alpha,12alpha-dihydroxy-5beta-cholestan-3-one + NADPH + H+ 1.1.1.213 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)
1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)


7alpha,25-Dihydroxycholesterol + NAD+ <=> 7alpha,25-dihydroxy-4-cholesten-3-one + NADH 1.1.1.145 3beta-hydroxy-Delta5-steroid dehydrogenase
1.1.1.181 cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase


25-hydroxycholesterol + flavin_hydroquinone + O2 <=> 7alpha,25-Dihydroxycholesterol + [oxidized_NADPH-hemoprotein_reductase] + H2O 1.14.14.29 25/26-hydroxycholesterol 7alpha-hydroxylase

(24E)-3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA + H2O <=> (24R,25R)-3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA 4.2.1.107 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase

7alpha,27-dihydroxycholesterol + NAD+ <=> 7alpha,27-dihydroxy-4-cholesten-3-one + NADH 1.1.1.145 3beta-hydroxy-Delta5-steroid dehydrogenase
1.1.1.181 cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase


propionyl-CoA + chenodeoxycholoyl-CoA <=> CoA + 3alpha,7alpha-Dihydroxy-5beta-24-oxocholestanoyl-CoA 2.3.1.176 propanoyl-CoA C-acyltransferase

glycocholate + H2O <=> cholic_acid + glycine 3.5.1.24 choloylglycine hydrolase

(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA + NAD+ <=> H+ + 3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholestanoyl-CoA + NADH 1.1.1

(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al + reduced_adrenodoxin + O2 + H+ <=> 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic_acid + oxidized_ferredoxin + H2O 1.14.15.15 cholestanetriol 26-monooxygenase
1.2.1.40 3alpha,7alpha,12alpha-trihydroxycholestan-26-al 26-oxidoreductase


5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced_adrenodoxin + H+ + O2 <=> (25R)-5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol + oxidized_ferredoxin + H2O 1.14.15.15 cholestanetriol 26-monooxygenase

5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol <=> 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al 1.14.15.15 cholestanetriol 26-monooxygenase

5-cholestene-26-oic_acid-3beta-ol + O2 + NADPH + H+ <=> 3beta,7alpha-dihydroxy-5-cholestenoic_acid + NADP+ + H2O 1.14.14.29 25/26-hydroxycholesterol 7alpha-hydroxylase

(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA <=> (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O 4.2.1.107 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase
4.2.1.119 enoyl-CoA hydratase 2


3alpha,7alpha-Dihydroxy-5beta-cholestan-26-al + O2 + 2 H+ + 2 reduced_ferredoxin <=> 3alpha,7alpha-dihydroxy-5beta-cholestanate + H2O + 2 oxidized_ferredoxin 1.14.15.15 cholestanetriol 26-monooxygenase

3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic_acid + ATP + CoA <=> trihydroxycoprostanoyl-CoA + AMP + diphosphate 6.2.1.7 cholate---CoA ligase

7alpha-hydroxycholesterol + NAD+ <=> 7alpha-hydroxy-4-cholesten-3-one + NADH + H+ 1.1.1.145 3beta-hydroxy-Delta5-steroid dehydrogenase
1.1.1.181 cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase


taurocholate + H2O <=> cholic_acid + taurine 3.5.1
3.5.1.24 choloylglycine hydrolase
3.5.1.74 chenodeoxycholoyltaurine hydrolase



ATP + cholic_acid + CoA <=> AMP + diphosphate + choloyl-CoA 6.2.1.7 cholate---CoA ligase

(24S)-cholest-5-ene-3beta,7alpha,24-triol + NAD+ <=> 7alpha,24-Dihydroxy-4-cholesten-3-one + NADH + H+ 1.1.1.181 cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase

27-Hydroxycholesterol + 2 O2 + 2 NADPH + 2 H+ <=> 5-cholestene-26-oic_acid-3beta-ol + 2 NADP+ + 3 H2O not assigned

7alpha,12alpha-dihydroxy-4-cholesten-3-one + NADPH + H+ <=> 7alpha,12alpha-dihydroxy-5beta-cholestan-3-one + NADP+ 1.3.1.3 Delta4-3-oxosteroid 5beta-reductase

(25S)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA <=> trihydroxycoprostanoyl-CoA 5.1.99.4 alpha-methylacyl-CoA racemase

chenodeoxycholoyl-CoA + taurine <=> H+ + taurochenodeoxycholate + CoA 2.3.1.65 bile acid-CoA:amino acid N-acyltransferase

cholesterol + flavin_hydroquinone + O2 <=> (24S)-24-hydroxycholesterol + [oxidized_NADPH-hemoprotein_reductase] + H2O 1.14.14.25 cholesterol 24-hydroxylase

7alpha-hydroxy-4-cholesten-3-one + reduced_cytochrome_b5 + H+ + O2 <=> 7alpha,12alpha-dihydroxy-4-cholesten-3-one + ferricytochrome_b5 + H2O 1.14.18.8 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase

cholesterol + reduced_acceptor + O2 <=> 25-hydroxycholesterol + acceptor + H2O 1.14.99.38 cholesterol 25-hydroxylase

ATP + (25R)-3alpha,7alpha-dihydroxy-5-beta-cholestanate + CoA <=> AMP + diphosphate + 3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA 6.2.1.28 3alpha,7alpha-dihydroxy-5beta-cholestanate---CoA ligase
6.2.1.7 cholate---CoA ligase


(25S)-3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA <=> 3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA 5.1.99.4 alpha-methylacyl-CoA racemase

5beta-cholestane-3alpha,7alpha,26-triol <=> 3alpha,7alpha-Dihydroxy-5beta-cholestan-26-al 1.14.15.15 cholestanetriol 26-monooxygenase

choloyl-CoA + H2O <=> H+ + cholic_acid + CoA 2.8.3.25 bile acid CoA-transferase
3.1.2.2 palmitoyl-CoA hydrolase
3.1.2.20 acyl-CoA hydrolase
3.1.2.27 choloyl-CoA hydrolase




(24S)-24-hydroxycholesterol + flavin_hydroquinone + O2 <=> (24S)-cholest-5-ene-3beta,7alpha,24-triol + [oxidized_NADPH-hemoprotein_reductase] + H2O 1.14.14.26 24-hydroxycholesterol 7alpha-hydroxylase

7alpha,27-dihydroxy-4-cholesten-3-one + 2 O2 + 2 NADPH + 2 H+ <=> 7alpha-Hydroxy-3-oxo-4-cholestenoate + 2 NADP+ + 3 H2O not assigned

chenodeoxycholoyl-CoA + glycine <=> H+ + glycochenodeoxycholic_acid + CoA 2.3.1.65 bile acid-CoA:amino acid N-acyltransferase

5beta-cholestane-3alpha,7alpha-diol + flavin_hydroquinone + O2 <=> 5beta-cholestane-3alpha,7alpha,12alpha-triol + [oxidized_NADPH-hemoprotein_reductase] + H2O 1.14.14.139 5beta-cholestane-3alpha,7alpha-diol 12alpha-hydroxylase

glycochenodeoxycholic_acid + H2O <=> chenodeoxycholic_acid + glycine 3.5.1.24 choloylglycine hydrolase

5beta-cholestane-3alpha,7alpha-diol + NADP+ <=> 7alpha-hydroxy-5beta-cholestan-3-one + NADPH + H+ 1.1.1.213 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)
1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)


(25S)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + acceptor <=> (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + reduced_acceptor 1.17.99.3 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA 24-hydroxylase
1.3.99


choloyl-CoA + taurine <=> H+ + taurocholate + CoA 2.3.1.65 bile acid-CoA:amino acid N-acyltransferase

choloyl-CoA + propionyl-CoA <=> CoA + 3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholestanoyl-CoA 2.3.1.16 acetyl-CoA C-acyltransferase
2.3.1.176 propanoyl-CoA C-acyltransferase


taurochenodeoxycholate + H2O <=> chenodeoxycholic_acid + taurine 3.5.1.24 choloylglycine hydrolase
3.5.1.74 chenodeoxycholoyltaurine hydrolase


5beta-cholestane-3alpha,7alpha-diol + reduced_adrenodoxin + O2 <=> 5beta-cholestane-3alpha,7alpha,26-triol + oxidized_ferredoxin + H2O 1.14.15.15 cholestanetriol 26-monooxygenase

(24R,25R)-3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA + NAD+ <=> 3alpha,7alpha-Dihydroxy-5beta-24-oxocholestanoyl-CoA + NADH + H+ 1.1.1

5beta-cholestane-3alpha,7alpha-diol + NAD+ <=> 7alpha-hydroxy-5beta-cholestan-3-one + NADH + H+ 1.1.1.213 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)
1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)


5beta-cholestane-3alpha,7alpha,12alpha-triol + NAD+ <=> 7alpha,12alpha-dihydroxy-5beta-cholestan-3-one + NADH + H+ 1.1.1.213 3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)
1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)


3beta,7alpha-dihydroxy-5-cholestenoic_acid + NAD+ <=> 7alpha-Hydroxy-3-oxo-4-cholestenoate + NADH + H+ 1.1.1.181 cholest-5-ene-3beta,7alpha-diol 3beta-dehydrogenase

5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol <=> 5beta-cyprinolsulfate not assigned

choloyl-CoA + glycine <=> H+ + glycocholate + CoA 2.3.1.65 bile acid-CoA:amino acid N-acyltransferase

ATP + chenodeoxycholic_acid + CoA <=> AMP + diphosphate + chenodeoxycholoyl-CoA 6.2.1.7 cholate---CoA ligase

cholesterol + flavin_hydroquinone + O2 <=> 7alpha-hydroxycholesterol + [oxidized_NADPH-hemoprotein_reductase] + H2O 1.14.14.23 cholesterol 7alpha-monooxygenase

7alpha-hydroxy-4-cholesten-3-one + NADPH + H+ <=> 7alpha-hydroxy-5beta-cholestan-3-one + NADP+ 1.3.1.3 Delta4-3-oxosteroid 5beta-reductase

chenodeoxycholoyl-CoA + H2O <=> chenodeoxycholic_acid + CoA + H+ 3.1.2.2 palmitoyl-CoA hydrolase
3.1.2.20 acyl-CoA hydrolase
3.1.2.27 choloyl-CoA hydrolase



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